News has also circulated about a "sci-fi twist", due to information from developer interviews, and some effects visible in trailers of the game. It is theorized that the historical setting may be a simulation, or perhaps some sort of genetic memory, for purposes of training or other gain, being undertaken by an unknown group in the present or near future. During a trailer shown at E3, the screen would glitch and go fuzzy at times, and at one point where Altaïr is killed, the screen fades to black and then shows a head's up display with the words "computer offline" with a woman in a labcoat behind the display. Trailers shown at E3 07 reveal further evidence of this, such as a futuristic looking health bar, effects resembling readouts surrounding locked-on enemies and a rather lengthy conversation with a dying enemy surrounded by a white background. Ubisoft has confirmed that Assassin's Creed will be part of a trilogy, and has also stated that one or both of the sequels may have a futuristic setting.

In December 13, 2006, in an interview with IGN, Kristen Bell (who will have her voice and likeness lent to the game) talked about the plot. According to the interview, the plot centers on genetic memory and a corporation looking for descendants of an assassin.[4]
“ It's actually really interesting to me. It's sort of based on the research that's sort of happening now, about the fact that your genes might be able to hold memory. And you could argue semantics and say it's instinct, but how does a baby bird know to eat a worm, as opposed to a cockroach, if its parents don't show it? And it's about this science company trying to, Matrix-style, go into people's brains and find out an ancestor who used to be an assassin, and sort of locate who that person is.